Real Time Reverse Transcription Polymerase Chain Reaction (Real Time RT PCR)

Real‑Time Reverse Transcription Polymerase Chain Reaction (Real‑Time RT‑PCR) is a highly sensitive and specific molecular technique used for the real‑time detection and quantification of viral RNA in biological and environmental samples. The assay combines reverse transcription of viral RNA into complementary DNA (cDNA) with continuous monitoring of target amplification through fluorescence‑based detection using virus‑specific primers and probes. In VisMederi, Real‑Time RT‑PCR is widely applied for virus detection and quantitation from clinical specimens. The real‑time format enables precise quantification of viral genome copy number, supporting viral load determination, and particle‑to‑infectivity ratio assessments when integrated with infectivity assays. VisMederi’s Real‑Time RT‑PCR methods are developed, validated, and qualified in accordance with European Medicines Agency (EMA) and ICH guidelines for analytical performance, including specificity, sensitivity, accuracy, precision, and robustness, making Real‑Time RT‑PCR a critical tool for viral characterization, quality control, and regulatory submissions in both clinical and non‑clinical settings.