Cell-Mediated Immunity
Validation of a double-color ELISpot assay of IFN-γ and IL-4 production in human peripheral blood mononuclear cells.
Dapporto F, De Tommaso D, Marrocco C, Piu P, Semplici C, Fantoni G, Ferrigno I, Piccini G, Monti M, Vanni F, Razzano I, Manini I, Montomoli E, Manenti A. J Immunol Methods. 2024 Jan;524:113588. doi: 10.1016/j.jim.2023.113588. Epub 2023 Nov 29. PMID: 38040193.
Proficiency tests to evaluate the impact on assay outcomes of harmonized influenza-specific Intracellular Cytokine Staining (ICS) and IFN-γ Enzyme-Linked ImmunoSpot (ELISpot) protocols.
Waerlop G, Leroux-Roels G, Pagnon A, Begue S, Salaun B, Janssens M, Medaglini D, Pettini E, Montomoli E, Gianchecchi E, Lambe T, Godfrey L, Bull M, Bellamy D, Amdam H, Bredholt G, Cox RJ, Clement F. J Immunol Methods. 2023 Dec;523:113584. doi: 10.1016/j.jim.2023.113584. Epub 2023 Oct 31. PMID: 37918618.
Flow cytometry as an integrative method for the evaluation of vaccine immunogenicity: A validation approach.
Gianchecchi E, Torelli A, Piu P, Bonifazi C, Ganfini L, Montomoli E. Biochem Biophys Rep. 2023 Apr 22;34:101472. doi: 10.1016/j.bbrep.2023.101472. eCollection 2023 Jul. PMID: 37153861.
